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IB3008 METABOLIC ENGINEERING SYLLABUS | ANNA UNIVERSITY BTECH FOOD TECHNOLOGY 6TH SEMESTER SYLLABUS REGULATION 2008 2011 2012-2013

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IB3008 METABOLIC ENGINEERING SYLLABUS | ANNA UNIVERSITY BTECH FOOD TECHNOLOGY 6TH SEMESTER SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY SIXTH SEMESTER B.TECH FOOD TECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009

IB3008 METABOLIC ENGINEERING LT P C
3 0 0 3
UNIT I INTRODUCTION TO EXAMPLES OF PATHWAY MANIPULATION -
QUALITATIVE TREATMENT 9
Enhancement of Product Yield and Productivity,Extension of substrate Range, Extension of
Product spectrum and Novel products, Improvement of Cellular properties, Xenobiotic
degradation.
UNIT II MATERIAL BALANCES AND DATA CONSISTENCY 9
Comprehensive models of cellular reactions; stoichiometry of cellular reactions, reaction
rates, dynamic mass balances, yield coefficients and linear rate equations, analysis of over
determined systems- identification of gross measurement errors. Introduction to MATLAB®
UNIT III METABOLIC FLUX ANALYSIS 9
Theory, overdetermined systems, underdetermined systems- linear programming, sensitivity
analysis, methods for the experimental determination of metabolic fluxes by isotope labeling,
applications of metabolic flux analysis.
UNIT IV METABOLIC CONTROL ANALYSIS 9
Fundamentals of Metabolic Control Analysis, control coefficients and the summation
theorems, Determination of flux control coefficients, MCA of linear pathways, branched
pathways, theory of large deviations
66
UNIT V ANALYSIS OF METABOLIC NETWORKS 9
Control of flux distribution at a single branch point, Grouping of reactions, case studies,
extension of control analysis to intermetabolite, optimization of flux amplifications,
consistency tests and experimental validation.
TOTAL : 45 PERIODS
TEXT BOOKS
1. Stephanopoulos, G.N. “Metabolic Engineering : Principles and Methodologies”.
Academic Press / Elsevier, 1998.
2. Lee, S.Y. and Papoutsakis, E.T. “Metabolic Engineering”. Marcel Dekker, 1998.
3. Nielsen, J. and Villadsen, J. “Bioreaction Engineering Principles”. Springer, 2007.
REFERENCES
1. Voit, E.O. “Computational Analysis of Biochemical Systems : A Practical Guide for
Biochemists and Molecular Biologists”. Cambridge University Press, 2000.
2. Scheper, T. “Metabolic Engineering” Vol 73 (Advances in Biochemical Engineering
Biotechnology) Springer, 2001.
3. Rhodes, P.M. and P.F. Stanbury “Applied Microbial Physiology: Practical Approach”.
IRL Press, 1997.
4. Caldwell, D.R. “Microbial Physiology & Metabolism”. Wm. C. Brown, 1995.
5. Rehm, H.J. and G. Reed, “Biotechnology : Products of Primary Metabolism Vol.6 and
Biotechnology : Products of Secondary Metabolism Vol.7, VCH / Wiley, 1997.

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