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Monday, October 22, 2012

BT2254 INSTRUMENTAL METHODS OF ANALYSIS SYLLABUS | ANNA UNIVERSITY BTECH BIOTECHNOLOGY 4TH SEM SYLLABUS REGULATION 2008 2011 2012-2013

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BT2254 INSTRUMENTAL METHODS OF ANALYSIS SYLLABUS | ANNA UNIVERSITY BTECH BIOTECHNOLOGY 4TH SEM SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY FOURTH SEMESTER B.TECH BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009

BT2254 INSTRUMENTAL METHODS OF ANALYSIS L T P C
3 0 0 3
AIM(S) OF THE COURSE
The course aims to develop the skills of the students in the area of Instrumentation in
Biotechnology. This will be prerequisite for understanding specialized courses & project
work that will be offered in the subsequent semesters.
UNIT I BASICS OF MEASUREMENT 5
Classification of methods – calibration of instrumental methods – electrical components
and circuits – signal to noise ratio – signal – noise enhancement.
UNIT II OPTICAL METHODS 5
33
General design – sources of radiation – wavelength selectors – sample containers –
radiation transducers – types of optical instruments – Fourier transform measurements.
UNIT III MOLECULAR SPECTROSCOPY 15
Measurement of transmittance and absorbance – beer's law – spectrophotometer
analysis – qualitative and quantitative absorption measurements - types of
spectrometers – UV – visible – IR – Raman spectroscopy – instrumentation – theory.
UNIT IV THERMAL METHODS 5
Thermo-gravimetric methods – differential thermal analysis – differential scanning
calorimetry.
UNIT V SEPARATION METHODS 15
Introduction to chromatography – models – ideal separation – retention parameters –
van – deemter equation – gas chromatography – stationary phases – detectors – kovats
indices – HPLC – pumps – columns – detectors – ion exchange chromatography – size
exclusion chromatography – supercritical chromatography – capillary electrophoresis
TOTAL: 45 PERIODS
TEXT BOOKS
1. Instrumental Methods of Analysis; Willard and .H. Merrit, Phi, 1999.
2. Instrumental Methods of Analysis, D. Skoog, 2000.

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