BT2357 GENETIC ENGINEERING LAB SYLLABUS | ANNA UNIVERSITY BTECH BIOTECHNOLOGY 6TH SEMESTER SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY SIXTH SEMESTER B.TECH BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT2357 GENETIC ENGINEERING LABORATORY L T P C
0 0 4 2
AIM
To provide hands on training in the Genetic Engineering by the designing simple
experiments. This is a pre-requisite for Down-stream processing has offered in later
semester.
OBJECTIVES
At the end of the course, the student would have learnt about the cloning of genes,
how to express them for protein production & subsequent purification of protein. This
will be needed for any project work in modern biology.
1. Preparation of plasmid DNA.
2. Elution of DNA from agarose gels.
3. Ligation of DNA into expression vectors.
4. Transformation.
5. Optimisation of inducer concentration for recombinant protein expression.
6. Optimisation of time of inducer for recombinant protein expression.
7. SDS-PAGE, 2 D Gel, ISO – electric Focussing.
8. Western blotting.
9. Hybridisation with anti-sera.
10. PCR.
TOTAL : 60 PERIODS
BT2357 GENETIC ENGINEERING LABORATORY L T P C
0 0 4 2
AIM
To provide hands on training in the Genetic Engineering by the designing simple
experiments. This is a pre-requisite for Down-stream processing has offered in later
semester.
OBJECTIVES
At the end of the course, the student would have learnt about the cloning of genes,
how to express them for protein production & subsequent purification of protein. This
will be needed for any project work in modern biology.
1. Preparation of plasmid DNA.
2. Elution of DNA from agarose gels.
3. Ligation of DNA into expression vectors.
4. Transformation.
5. Optimisation of inducer concentration for recombinant protein expression.
6. Optimisation of time of inducer for recombinant protein expression.
7. SDS-PAGE, 2 D Gel, ISO – electric Focussing.
8. Western blotting.
9. Hybridisation with anti-sera.
10. PCR.
TOTAL : 60 PERIODS
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