BT2358 BIOPROCESS LAB SYLLABUS | ANNA UNIVERSITY BTECH BIOTECHNOLOGY 6TH SEMESTER SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY SIXTH SEMESTER B.TECH BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT2358 BIOPROCESS LAB L T P C 0 0 4 2
AIM
This course aims to provide hands a training in the laboratory of Bio process Technology
by performing simple experiments.
OBJECTIVES
At the end of the course, the student would have learnt about Bioreactors & how to
use them for practical applications. This will be beneficial to students to undertake
project work in this area.
1. Thermal death kinetics
2. Batch sterilization design
3. Batch cultivation, estimation of kla – dynamic gassing method, exhaust gas analysis
– carbon balancing, gas balancing
4. Batch and Fed batch cultivation, exhaust gas analysis – carbon balancing, gas
balancing
5. Total cell retention cultivation, exhaust gas analysis – carbon balancing, gas
balancing
6. Estimation of kla – sulphite oxidation method
7. Estimation of kla – power correlation method
8. Residence time distribution
9. Estimation of overall heat transfer coefficient
10. Continuous cultivation – x-d diagram, pulse and shift method, evaluation of kinetic
parameters, exhaust gas analysis – carbon balancing, gas balancing.
11. Enzyme kinetics – micheies menton parameters.
52
12. Enzyme immobilization – gee intropment & cross linking methods.
BT2358 BIOPROCESS LAB L T P C 0 0 4 2
AIM
This course aims to provide hands a training in the laboratory of Bio process Technology
by performing simple experiments.
OBJECTIVES
At the end of the course, the student would have learnt about Bioreactors & how to
use them for practical applications. This will be beneficial to students to undertake
project work in this area.
1. Thermal death kinetics
2. Batch sterilization design
3. Batch cultivation, estimation of kla – dynamic gassing method, exhaust gas analysis
– carbon balancing, gas balancing
4. Batch and Fed batch cultivation, exhaust gas analysis – carbon balancing, gas
balancing
5. Total cell retention cultivation, exhaust gas analysis – carbon balancing, gas
balancing
6. Estimation of kla – sulphite oxidation method
7. Estimation of kla – power correlation method
8. Residence time distribution
9. Estimation of overall heat transfer coefficient
10. Continuous cultivation – x-d diagram, pulse and shift method, evaluation of kinetic
parameters, exhaust gas analysis – carbon balancing, gas balancing.
11. Enzyme kinetics – micheies menton parameters.
52
12. Enzyme immobilization – gee intropment & cross linking methods.
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