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Tuesday, November 13, 2012

BT2355 GENETIC ENGINEERING SYLLABUS | ANNA UNIVERSITY BTECH BIOTECHNOLOGY 6TH SEMESTER SYLLABUS REGULATION 2008 2011 2012-2013

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BT2355 GENETIC ENGINEERING SYLLABUS | ANNA UNIVERSITY BTECH BIOTECHNOLOGY 6TH SEMESTER SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY SIXTH SEMESTER B.TECH BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009

BT2355 GENETIC ENGINEERING L T P C
3 0 0 3
AIM
To develop skills of the students in the area of genetic Engineering. This will be a prerequisite
for electives like genomics & proteomics, Immuno technology offered in the
subsequent semesters.
OBJECTIVES
 At the end of the course, the student would learnt about various aspects of genetic
engineering and its application This will be very useful for the student to undertake
research /project work in Modern Biology.
UNIT I BASICS OF RECOMBINANT DNA TECHNOLOGY 4
Role of genes within cells, genetic elements that control gene expression, restriction and
modifying enzymes, safety guidelines of recombinant DNA research.
UNIT II CREATION OF RECOMBINANT MOLECULES 10
Restriction mapping, design of linkers and adaptors. Characteristics of plasmid and
phage vectors, prokaryotic and eukaryotic expression vectors. Insect, Yeast and
Mammalian vectors.
UNIT III CONSTRUCTION OF LIBRARIES 15
Construction of cDNA and genomic libraries. Screening of libraries with DNA probes and
with antisera.
UNIT IV POLYMERASE CHAIN REACTION 10
Inverse PCR, Nested PCR, Taqman assay, Molecular beacons, RACE PCR, RAPD, site
directed mutagenesis, methods of nucleic acid sequencing- Sangers method, (Kunkel’s
Method).
UNIT V APPLICATIONS OF RECOMBINANT DNA TECHNOLOGY 6
Cloning in plants, Ti plasmid, and transgenic and knockout animals.
TOTAL : 45 PERIODS
TEXT BOOKS
1. Old RW, Primrose SB, “Principles of Gene Manipulation, An Introduction To Genetic
Engineering”, Blackwell Science Publications, 1993.
2. Ansubel FM, Brent R, Kingston RE, Moore DD, “Current Protocols In Molecular
Biology”, Greene Publishing Associates, NY, 1988.
REFERENCES
1. Berger Sl, Kimmer AR, “Methods In Enzymology”, Vol. 152, Academic Press, 1987.

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