BT 9252 ENZYME TECHNOLOGY AND INDUSTRIAL APPLICATIONS SYLLABUS | ANNA UNIVERSITY MTECH BIOTECHNOLOGY 1ST SEM SYLLABUS REGULATION 2009 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY FIRST SEMESTER M.TECH BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2009 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT 9252 ENZYME TECHNOLOGY AND INDUSTRIAL APPLICATIONS L T P C
3 0 0 3
UNIT I KINETICS AND MECHANISM OF ENZYME ACTION 8
Classification of enzymes; quantification of enzyme activity and specific activity.
Estimation of Michaelis Menten parameters, Effect of pH and temperature on enzyme
activity, kinetics of inhibition. Modeling of rate equations for single and multiple substrate
reactions.
UNIT II IMMOBILISED ENZYME REACTIONS 9
Techniques of enzyme immobilisation-matrix entrapment, ionic and cross linking, column
packing; Analysis of mass transfer effects of kinetics of immobilised enzyme reactions;
Analysis of Film and Pore Diffusion Effects on Kinetics of immobilized enzyme reactions;
calculation of Effectiveness Factors of immobilized enzyme systems; Bioconversion
studies with immobilized enzyme packed -bed reactors.
UNIT III MASS TRANSFER EFFECTS IN IMMOBILISED ENZYME SYSTEMS
5
Analysis of film and Pore diffusion Effects on kinetics of immobilised enzyme reactions;
Formulation of dimensionless groups and calculation of Effectiveness Factors
UNIT IV APPLICATION OF ENZYMES 12
Extraction of commercially important enzymes from natural sources; Commercial
applications of enzymes in food, pharmaceutical and other industries; enzymes for
diagnostic applications. Industrial production of enzymes. Use of enzymes in analysistypes
of sensing-gadgetry and methods. Case studies on application - chiral
conversion, esterification etc.,
UNIT V ENZYME BIOSENSORS 11
Applications of enzymes in analysis; Design of enzyme electrodes and case studies on
their application as biosensors in industry, healthcare and environment.
TOTAL: 45 PERIODS
REFERENCES
1. Blanch, H.W., Clark, D.S. Biochemical Engineering, Marcel Dekker, 1997 Lee,
James M. Biochemical Engineering, PHI, USA.
2. Bailey J.E. & Ollis, D.F. Biochemical Engineering Fundamentals, 2nd Ed., McGraw
Hill, 1986
3. Wiseman, Alan. Hand book of Enzyme Biotechnology, 3rd ed., Ellis Harwood
1995.
BT 9252 ENZYME TECHNOLOGY AND INDUSTRIAL APPLICATIONS L T P C
3 0 0 3
UNIT I KINETICS AND MECHANISM OF ENZYME ACTION 8
Classification of enzymes; quantification of enzyme activity and specific activity.
Estimation of Michaelis Menten parameters, Effect of pH and temperature on enzyme
activity, kinetics of inhibition. Modeling of rate equations for single and multiple substrate
reactions.
UNIT II IMMOBILISED ENZYME REACTIONS 9
Techniques of enzyme immobilisation-matrix entrapment, ionic and cross linking, column
packing; Analysis of mass transfer effects of kinetics of immobilised enzyme reactions;
Analysis of Film and Pore Diffusion Effects on Kinetics of immobilized enzyme reactions;
calculation of Effectiveness Factors of immobilized enzyme systems; Bioconversion
studies with immobilized enzyme packed -bed reactors.
UNIT III MASS TRANSFER EFFECTS IN IMMOBILISED ENZYME SYSTEMS
5
Analysis of film and Pore diffusion Effects on kinetics of immobilised enzyme reactions;
Formulation of dimensionless groups and calculation of Effectiveness Factors
UNIT IV APPLICATION OF ENZYMES 12
Extraction of commercially important enzymes from natural sources; Commercial
applications of enzymes in food, pharmaceutical and other industries; enzymes for
diagnostic applications. Industrial production of enzymes. Use of enzymes in analysistypes
of sensing-gadgetry and methods. Case studies on application - chiral
conversion, esterification etc.,
UNIT V ENZYME BIOSENSORS 11
Applications of enzymes in analysis; Design of enzyme electrodes and case studies on
their application as biosensors in industry, healthcare and environment.
TOTAL: 45 PERIODS
REFERENCES
1. Blanch, H.W., Clark, D.S. Biochemical Engineering, Marcel Dekker, 1997 Lee,
James M. Biochemical Engineering, PHI, USA.
2. Bailey J.E. & Ollis, D.F. Biochemical Engineering Fundamentals, 2nd Ed., McGraw
Hill, 1986
3. Wiseman, Alan. Hand book of Enzyme Biotechnology, 3rd ed., Ellis Harwood
1995.
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