BT 9225 MICROBIAL AND IMMUNO TECHNOLOGY LAB SYLLABUS | ANNA UNIVERSITY MTECH BIOTECHNOLOGY 1ST SEM SYLLABUS REGULATION 2009 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY FIRST SEMESTER M.TECH BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2009 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT 9225 MICROBIAL AND IMMUNO TECHNOLOGY LAB L T P C 0 0 6 3
1. Sterilization, disinfection, safety in microbiological laboratory.
2. Preparation of media for growth of various microorganisms.
3. Identification and culturing of various microorganisms.
4. Staining and enumeration of microorganisms.
5. Growth curve, measure of bacterial population by turbidometry and studying the effect of temperature, pH, carbon and nitrogen.
6. Selection of animals, Preparation of antigens, Immunization and methods of bleeding, Serum separation, Storage.
7. Antibody titre by ELISA method.
8. Double diffusion, Immuno-electrophoresis and Radial Immuno diffusion.
9. SDS-PAGE, Immunoblotting, Dot blot assays
10. Blood smear identification of leucocytes by Giemsa stain
11. Separation of mononuclear cells by Ficoll-Hypaque
12. Immunodiagnostics using commercial kits
TOTAL : 90 PERIODS
BT 9225 MICROBIAL AND IMMUNO TECHNOLOGY LAB L T P C 0 0 6 3
1. Sterilization, disinfection, safety in microbiological laboratory.
2. Preparation of media for growth of various microorganisms.
3. Identification and culturing of various microorganisms.
4. Staining and enumeration of microorganisms.
5. Growth curve, measure of bacterial population by turbidometry and studying the effect of temperature, pH, carbon and nitrogen.
6. Selection of animals, Preparation of antigens, Immunization and methods of bleeding, Serum separation, Storage.
7. Antibody titre by ELISA method.
8. Double diffusion, Immuno-electrophoresis and Radial Immuno diffusion.
9. SDS-PAGE, Immunoblotting, Dot blot assays
10. Blood smear identification of leucocytes by Giemsa stain
11. Separation of mononuclear cells by Ficoll-Hypaque
12. Immunodiagnostics using commercial kits
TOTAL : 90 PERIODS
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