BT 9224 ANIMAL BIOTECHNOLOGY SYLLABUS | ANNA UNIVERSITY MTECH BIOTECHNOLOGY 1ST SEM SYLLABUS REGULATION 2009 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY FIRST SEMESTER M.TECH BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2009 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT 9224 ANIMAL BIOTECHNOLOGY L T P C
3 0 0 3
UNIT I INTRODUCTION 4
Scope of Animal Biotechnology, Animal Biotechnology for production of regulatory
proteins, blood products, vaccines, hormones and other therapeutic proteins.
UNIT II MOLECULAR BIOLOGY 9
Biology of animal viral vectors- SV40, adeno virus, retrovirus, vaccinia virus, herpes
virus, adeno associated virus and baculo virus.
UNIT III CELL CULTURE TECHNOLOGY 11
Culturing of cells, primary and secondary cell lines, Cell culture-Scaling up of animal cell
culture-monolayer culture, suspension culture; Various bio-reactors used for animal cell
culture-Roller bottle culture; Bioreactor process control, stirred animal cell culture, Air-lift
fermentor, Chemostat/Turbidostat; High technology vaccines; Hybridoma technology;
Cell lines and their applications
UNIT IV GENETIC ENGINEERING 11
Gene therapy-prospects and problems; Knock out mice and mice model for human
genetic disorder; Baculo virus in biocontrol; Enzymes technology, Somatic manipulation
of DNA, Nucleic acid hybridization and probes in diagnosis- preparation of probes,
evaluation and applications.
UNIT V APPLICATIONS 10
Rumen manipulation- probiotics embryo transfer technology, invitro fertilization,
transgenesis- methods of transferring genes into animal oocytes, eggs, embryos and
specific tissues by physical, chemical and biological methods; Biopharming -Transgenic
animals (Mice, Cows, Pigs, Sheep, Goat, Birds and Insects); Artificial insemination and
embryo transfer.
TOTAL : 45 PERIODS
REFERENCES
1. Watson, J.D., Gilman, M., Witowski J.and Zoller, M. Recombinant DNA, 2nd ed.,
Scientific American Books, 1983
2. Glick, B.R. and Pasternack, J.J. Molecular Biotechnology, 3rd ed., ASM Press, 2003
3. Lewin, B. Genes VIII , Pearson Prentice Hall, 2004
4. Davis J.M. Basic Cell Culture: A Practical Approach, IRL Press, 1998
5. Freshney R.I. Animal Cell Culture- a practical approach, 1987
BT 9224 ANIMAL BIOTECHNOLOGY L T P C
3 0 0 3
UNIT I INTRODUCTION 4
Scope of Animal Biotechnology, Animal Biotechnology for production of regulatory
proteins, blood products, vaccines, hormones and other therapeutic proteins.
UNIT II MOLECULAR BIOLOGY 9
Biology of animal viral vectors- SV40, adeno virus, retrovirus, vaccinia virus, herpes
virus, adeno associated virus and baculo virus.
UNIT III CELL CULTURE TECHNOLOGY 11
Culturing of cells, primary and secondary cell lines, Cell culture-Scaling up of animal cell
culture-monolayer culture, suspension culture; Various bio-reactors used for animal cell
culture-Roller bottle culture; Bioreactor process control, stirred animal cell culture, Air-lift
fermentor, Chemostat/Turbidostat; High technology vaccines; Hybridoma technology;
Cell lines and their applications
UNIT IV GENETIC ENGINEERING 11
Gene therapy-prospects and problems; Knock out mice and mice model for human
genetic disorder; Baculo virus in biocontrol; Enzymes technology, Somatic manipulation
of DNA, Nucleic acid hybridization and probes in diagnosis- preparation of probes,
evaluation and applications.
UNIT V APPLICATIONS 10
Rumen manipulation- probiotics embryo transfer technology, invitro fertilization,
transgenesis- methods of transferring genes into animal oocytes, eggs, embryos and
specific tissues by physical, chemical and biological methods; Biopharming -Transgenic
animals (Mice, Cows, Pigs, Sheep, Goat, Birds and Insects); Artificial insemination and
embryo transfer.
TOTAL : 45 PERIODS
REFERENCES
1. Watson, J.D., Gilman, M., Witowski J.and Zoller, M. Recombinant DNA, 2nd ed.,
Scientific American Books, 1983
2. Glick, B.R. and Pasternack, J.J. Molecular Biotechnology, 3rd ed., ASM Press, 2003
3. Lewin, B. Genes VIII , Pearson Prentice Hall, 2004
4. Davis J.M. Basic Cell Culture: A Practical Approach, IRL Press, 1998
5. Freshney R.I. Animal Cell Culture- a practical approach, 1987
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