BT 9221 BIOSEPARATION TECHNOLOGY SYLLABUS | ANNA UNIVERSITY MTECH BIOTECHNOLOGY 1ST SEM SYLLABUS REGULATION 2009 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY FIRST SEMESTER M.TECH BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2009 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT 9221 BIOSEPARATION TECHNOLOGY L T P C
3 0 0 3
UNIT I INTRODUCTION TO BIOSEPARATION 4
Characterization of biomolecules and fermentation broth. Guidelines to recombinant
protein purification.
UNIT II SOLID-LIQUID SEPARATION AND CELL DISRUPTION 6
Solid liquid separation- microfiltration and centrifugation – theory and design for scaleup
operation. Cell disruption – Homogeniser , dynomill – principle, factors affecting
disruption, batch and continuous operation. Cell disruption by chemical methods.
UNIT III CONCENTRATION AND PURIFICATION 7
Liq- liq extraction – theory and practice with emphasis on Aqueous two phase extraction.
Solid liquid extraction. Precipitation techniques using salt and solvent. Separation by
ultrafiltration, Dialysis, Electrophoresis.
UNIT IV CHROMATOGRAPHY 15
Theory, practice and selection of media for – Gelfiltration chromatography, Ion exchange
chromatography, Hydrophobic interaction chromatography, reverse phase
chromatography, Affinity chromatography – Metal affinity chromatography, dye affinity
chromatography, immunosorbent affinity chromatography & Expanded bed
chromatography. Scaleup criteria for chromatography, calculation of no of theoretical
plates and design
UNIT V FINAL POLISHING AND CASE STUDIES 13
Freeze drying, spray drying and crystallization. Purification of cephalosporin, aspartic
acid, Recombinant Streptokinase, Monoclonal antibodies, Tissue plasminogen activator,
Taq polymerase, Insulin.
TOTAL : 45 PERIODS
REFERENCES
1. Belter,P.A. et al., Bioseparations: Downstream Processing For Biotechnology, John-
Wiley , 1988
2. Janson J.C, & Ryden L. Protein Purification: Principles, High Resolution Methods
And Applications, VCH Pub. 1989.
3. Scopes R.K. – Protein Purification – Principles And Practice, Narosa , 1994.
BT 9221 BIOSEPARATION TECHNOLOGY L T P C
3 0 0 3
UNIT I INTRODUCTION TO BIOSEPARATION 4
Characterization of biomolecules and fermentation broth. Guidelines to recombinant
protein purification.
UNIT II SOLID-LIQUID SEPARATION AND CELL DISRUPTION 6
Solid liquid separation- microfiltration and centrifugation – theory and design for scaleup
operation. Cell disruption – Homogeniser , dynomill – principle, factors affecting
disruption, batch and continuous operation. Cell disruption by chemical methods.
UNIT III CONCENTRATION AND PURIFICATION 7
Liq- liq extraction – theory and practice with emphasis on Aqueous two phase extraction.
Solid liquid extraction. Precipitation techniques using salt and solvent. Separation by
ultrafiltration, Dialysis, Electrophoresis.
UNIT IV CHROMATOGRAPHY 15
Theory, practice and selection of media for – Gelfiltration chromatography, Ion exchange
chromatography, Hydrophobic interaction chromatography, reverse phase
chromatography, Affinity chromatography – Metal affinity chromatography, dye affinity
chromatography, immunosorbent affinity chromatography & Expanded bed
chromatography. Scaleup criteria for chromatography, calculation of no of theoretical
plates and design
UNIT V FINAL POLISHING AND CASE STUDIES 13
Freeze drying, spray drying and crystallization. Purification of cephalosporin, aspartic
acid, Recombinant Streptokinase, Monoclonal antibodies, Tissue plasminogen activator,
Taq polymerase, Insulin.
TOTAL : 45 PERIODS
REFERENCES
1. Belter,P.A. et al., Bioseparations: Downstream Processing For Biotechnology, John-
Wiley , 1988
2. Janson J.C, & Ryden L. Protein Purification: Principles, High Resolution Methods
And Applications, VCH Pub. 1989.
3. Scopes R.K. – Protein Purification – Principles And Practice, Narosa , 1994.
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