BT 9214 PREPARATIVE AND ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY SYLLABUS | ANNA UNIVERSITY MTECH BIOTECHNOLOGY 1ST SEM SYLLABUS REGULATION 2009 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY FIRST SEMESTER M.TECH BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2009 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT 9214 PREPARATIVE AND ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY L T P C
0 0 6 3
1. Preparation of Acetate, Tris and Phosphate Buffer systems and validation of
Henderson-Hasselbach equation.
2. Reactions of amino acids – Ninhydrin, Pthaldehyde, Dansyl chloride –
measurement using colorimetric and fluorimetric methods.
3. Differential estimations of carbohydrates – reducing vs non-reducing,
polymeric vs oligomeric, hexose vs pentose
4. Estimation of protein concentration using Lowrys’ method, Dye-binding method
5. DNA determination by UV-Vis Spectrophotometer – hyperchromic effect
6. Separation of lipids by TLC.
7. Enzyme Kinetics: Direct and indirect assays – determination of Km, Vmax and
Kcat, Kcat/ Km.
8. Restriction enzyme – Enrichment and unit calculation
9. Ion-exchange Chromatagraphy – Purification of IgG and Albumin
10. Gel filtration – Size based separation of proteins
11. Affinity chromatography – IMAC purification of His-tagged recombinant
protein
12. Assessing purity by SDS-PAGE Gel Electrophoresis
13. Chemical modification of proteins – PITC modification of IgG and Protein
immobilization
TOTAL : 90 PERIODS
REFERENCES
1. Biochemical Methods: A Concise Guide for Students and Researchers, Alfred
Pingoud, Claus Urbanke, Jim Hoggett, Albert Jeltsch, 2002 John Wiley & Sons
Publishers, Inc,
2. Biochemical Calculations: How to Solve Mathematical Problems in General
Biochemistry, 2nd Edition, Irwin H. Segel, 1976 John Wiley & Sons Publishers, Inc,
3. Principles and Techniques of Practical Biochemistry- Wilson, K. and Walker, J.
Cambridge Press.
BT 9214 PREPARATIVE AND ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY L T P C
0 0 6 3
1. Preparation of Acetate, Tris and Phosphate Buffer systems and validation of
Henderson-Hasselbach equation.
2. Reactions of amino acids – Ninhydrin, Pthaldehyde, Dansyl chloride –
measurement using colorimetric and fluorimetric methods.
3. Differential estimations of carbohydrates – reducing vs non-reducing,
polymeric vs oligomeric, hexose vs pentose
4. Estimation of protein concentration using Lowrys’ method, Dye-binding method
5. DNA determination by UV-Vis Spectrophotometer – hyperchromic effect
6. Separation of lipids by TLC.
7. Enzyme Kinetics: Direct and indirect assays – determination of Km, Vmax and
Kcat, Kcat/ Km.
8. Restriction enzyme – Enrichment and unit calculation
9. Ion-exchange Chromatagraphy – Purification of IgG and Albumin
10. Gel filtration – Size based separation of proteins
11. Affinity chromatography – IMAC purification of His-tagged recombinant
protein
12. Assessing purity by SDS-PAGE Gel Electrophoresis
13. Chemical modification of proteins – PITC modification of IgG and Protein
immobilization
TOTAL : 90 PERIODS
REFERENCES
1. Biochemical Methods: A Concise Guide for Students and Researchers, Alfred
Pingoud, Claus Urbanke, Jim Hoggett, Albert Jeltsch, 2002 John Wiley & Sons
Publishers, Inc,
2. Biochemical Calculations: How to Solve Mathematical Problems in General
Biochemistry, 2nd Edition, Irwin H. Segel, 1976 John Wiley & Sons Publishers, Inc,
3. Principles and Techniques of Practical Biochemistry- Wilson, K. and Walker, J.
Cambridge Press.
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