BT2203 BIOCHEMISTRY - I SYLLABUS | ANNA UNIVERSITY B.TECH. BIOTECHNOLOGY 3RD SEMESTER SYLLABUS REGULATION 2008 2011-2012 BELOW IS THE ANNA UNIVERSITY THIRD SEMESTER B.TECH. BIOTECHNOLOGY DEPARTMENT SYLLABUS IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011-2012 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI,TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT2203 BIOCHEMISTRY - I L T P C
3 0 0 3
AIM
To enable students learn the basic fundamental of biochemical Processes.
OBJECTIVE
To ensure students have a strong grounding in structures and reactions of
biomolecules.
To introduce them to metabolic pathway of the major bimolecular and relevance to
clinical conductors.
To correlate biochemical processes with biotechnology applications.
UNIT I INTRODUCTION TO BIOMOLECULES 5
Basic principles of organic chemistry, types of functional groups, biomolecules, chemical
nature, water, pH and biological buffers.
UNIT II STRUCTURE AND PROPERTIES 15
Structure and properties of Important Biomolecules.
Carbohydrates (mono, di, oligo & polysaccharides) Proteoglycans,
glucosaminoglycans. mutarotation, glycosidic bond, reactions of monosaccharides,
reducing sugars .
Lipids: fatty acids, glycerol, saponification, iodination, hydrogenation, phospholipids,
glycolipids, sphingolipids, cholesterol, steroids, prostaglandins.
Amino Acids, Peptides, Proteins, measurement, structures, hierarchy of organization
primary, secondary, tertiary and quaternary structures, glycoproteins, lipoproteins.
Determine of primary structure.
Nucleic acids: purines, pyrimidines, nucleoside, nucleotide, RNA, DNA ,reactions,
properties, measurement, nucleoprotein complexes
UNIT III METABOLISM CONCEPTS 5
Functions of Proteins, Enzymes, introduction to biocatalysts, metabolic pathways,
primary and secondary metabolites.
UNIT IV INTERMEDIARY METABOLISM AND REGULATION 15
Glycolysis, TCA cycle, gluconeogenesis, pentose phosphate shunt, glyoxalate shunt,
fatty acid synthesis and oxidation, reactions of amino acids, deamination, transamination
and decarboxylation, urea cycle, interconnection of pathways and metabolic regulation.
Case study on overproduction of glutamic acid, threonine , lysine, methionine, isoleucine
and ethanol.
UNIT V BIOENERGETICS 5
High energy compounds, electronegative potential of compounds, respiratory chain, ATP
cycle, calculation of ATP yield during oxidation of glucose and fatty acids.
27
TOTAL : 45 PERIODS
TEXT BOOKS
1. Nelson, D.L. and M.M. Cox, “Lehninger’s Principles of Biochemsitry”, 4th Edition,
W.H. Freemen & Co., 2005.
2. Stryer, L., “Biochemsitry”, 4th Edition, W.H. Freeman & Co., 2000.
3. Voet, D. and Voet, J.G., “Biochemistry”, 3rd Edition, John Wiley & Sons Inc., 2004.
4. Murray, R.K., etal “Harper’s Biochemistry”, 23rd Edition, Prentice Hall International,
1993.
BT2207 BIOCHEMISTRY LABORATORY L T P C
0 0 4 2
MAIN TOPICS OF STUDY
Demonstration of use of volume and weight measurements devices.
Titration of weak acid-weak base.
Quantitative Test for carbohydrates
Distinguish reducing and nontraducing sugars.
Using ninhydrin for distinguishing Imino and amino acids
Protein estimation by Biuret and Lowry’s methods.
Protein estimation by Bradfrd colorometric methods.
Extraction of lipids and analysis by TLC.
Estimation of nucleus ends by absorbance at 260mm and hyper chromicity.
Enzymatic assay of phosphates.
Hydrolysis of starch by an enzyme
TOTAL: 60 PERIODS
REFERENCES
1. Wilson and Walker “Principles and Techniques of Practical Biochemistry” 4 Edn.,
Cambridge Knew pros 1997.
2. Plummer DT “An Introduction to Practical Biochemistry” III Edn., Tata McGrawhill.
LIST OF EQUIPMENTS
Heating Mantles (5) / Water Baths (5) / Bunsen Buners (10)
TLC Plates – Required Numbers
Colorimeter – 2 Nos.
Consumables and Reagents.
BT 2208 BIOORGANIC CHEMISTRY LABORATORY L T P C
0 0 4 2
AIM(S) OF THE COURSE
The course aims is offering hands on training in the area of Bio Organic Chemistry. This
will be a prerequisite for certain lab courses offered in the subsequent semesters and
also for the project work.
28
LIST OF EXPERIMENTS
1. Synthesis of aspirin
2. Hydrolysis of sucrose
3. Preparation of pyruvic acid.
4. Preparation of oleic acid
5. Preparation of alpha D- glucopyranose pentaacetate
6. Preparation of 1,2,5,6 dicyclohexylnoine alpha D glucofuranose
7. Isolation of lycopene from tomato paste
8. Preparation of L-proline
9. Preparation of L-cysteine from hair
10. Preparation of S-ethyl hydroxybutonate from ethyl acetoacetate using yeast
11. Resolution of S-ethyl hydroxybutonate using 3,5 dinitrobenzoate.
12. Preparation of 5,10,15,20-tetrakisphenyl porphyrin.
TOTAL: 60 PERIODS
REFERENCE
1. Fummis B.S., Hannaford A.J., Smith P.W.G., “Text Book of Practical Organic
Chemistry”, Longman Edition, 1995.
EQUIPMENTS / APPARATUS REQUIREMENTS
Heating Mantles (Nos. 5) / Water baths (Nos. 5) / Bunsen Burners (Nos. 15)
Round bottom flasks of various volumes (100ml, 500 ml, 250 ml – Nos.5)
condensers (Nos. 5), Distillation units (Nos. 2).
Reagents and consumables.
BT2203 BIOCHEMISTRY - I L T P C
3 0 0 3
AIM
To enable students learn the basic fundamental of biochemical Processes.
OBJECTIVE
To ensure students have a strong grounding in structures and reactions of
biomolecules.
To introduce them to metabolic pathway of the major bimolecular and relevance to
clinical conductors.
To correlate biochemical processes with biotechnology applications.
UNIT I INTRODUCTION TO BIOMOLECULES 5
Basic principles of organic chemistry, types of functional groups, biomolecules, chemical
nature, water, pH and biological buffers.
UNIT II STRUCTURE AND PROPERTIES 15
Structure and properties of Important Biomolecules.
Carbohydrates (mono, di, oligo & polysaccharides) Proteoglycans,
glucosaminoglycans. mutarotation, glycosidic bond, reactions of monosaccharides,
reducing sugars .
Lipids: fatty acids, glycerol, saponification, iodination, hydrogenation, phospholipids,
glycolipids, sphingolipids, cholesterol, steroids, prostaglandins.
Amino Acids, Peptides, Proteins, measurement, structures, hierarchy of organization
primary, secondary, tertiary and quaternary structures, glycoproteins, lipoproteins.
Determine of primary structure.
Nucleic acids: purines, pyrimidines, nucleoside, nucleotide, RNA, DNA ,reactions,
properties, measurement, nucleoprotein complexes
UNIT III METABOLISM CONCEPTS 5
Functions of Proteins, Enzymes, introduction to biocatalysts, metabolic pathways,
primary and secondary metabolites.
UNIT IV INTERMEDIARY METABOLISM AND REGULATION 15
Glycolysis, TCA cycle, gluconeogenesis, pentose phosphate shunt, glyoxalate shunt,
fatty acid synthesis and oxidation, reactions of amino acids, deamination, transamination
and decarboxylation, urea cycle, interconnection of pathways and metabolic regulation.
Case study on overproduction of glutamic acid, threonine , lysine, methionine, isoleucine
and ethanol.
UNIT V BIOENERGETICS 5
High energy compounds, electronegative potential of compounds, respiratory chain, ATP
cycle, calculation of ATP yield during oxidation of glucose and fatty acids.
27
TOTAL : 45 PERIODS
TEXT BOOKS
1. Nelson, D.L. and M.M. Cox, “Lehninger’s Principles of Biochemsitry”, 4th Edition,
W.H. Freemen & Co., 2005.
2. Stryer, L., “Biochemsitry”, 4th Edition, W.H. Freeman & Co., 2000.
3. Voet, D. and Voet, J.G., “Biochemistry”, 3rd Edition, John Wiley & Sons Inc., 2004.
4. Murray, R.K., etal “Harper’s Biochemistry”, 23rd Edition, Prentice Hall International,
1993.
BT2207 BIOCHEMISTRY LABORATORY L T P C
0 0 4 2
MAIN TOPICS OF STUDY
Demonstration of use of volume and weight measurements devices.
Titration of weak acid-weak base.
Quantitative Test for carbohydrates
Distinguish reducing and nontraducing sugars.
Using ninhydrin for distinguishing Imino and amino acids
Protein estimation by Biuret and Lowry’s methods.
Protein estimation by Bradfrd colorometric methods.
Extraction of lipids and analysis by TLC.
Estimation of nucleus ends by absorbance at 260mm and hyper chromicity.
Enzymatic assay of phosphates.
Hydrolysis of starch by an enzyme
TOTAL: 60 PERIODS
REFERENCES
1. Wilson and Walker “Principles and Techniques of Practical Biochemistry” 4 Edn.,
Cambridge Knew pros 1997.
2. Plummer DT “An Introduction to Practical Biochemistry” III Edn., Tata McGrawhill.
LIST OF EQUIPMENTS
Heating Mantles (5) / Water Baths (5) / Bunsen Buners (10)
TLC Plates – Required Numbers
Colorimeter – 2 Nos.
Consumables and Reagents.
BT 2208 BIOORGANIC CHEMISTRY LABORATORY L T P C
0 0 4 2
AIM(S) OF THE COURSE
The course aims is offering hands on training in the area of Bio Organic Chemistry. This
will be a prerequisite for certain lab courses offered in the subsequent semesters and
also for the project work.
28
LIST OF EXPERIMENTS
1. Synthesis of aspirin
2. Hydrolysis of sucrose
3. Preparation of pyruvic acid.
4. Preparation of oleic acid
5. Preparation of alpha D- glucopyranose pentaacetate
6. Preparation of 1,2,5,6 dicyclohexylnoine alpha D glucofuranose
7. Isolation of lycopene from tomato paste
8. Preparation of L-proline
9. Preparation of L-cysteine from hair
10. Preparation of S-ethyl hydroxybutonate from ethyl acetoacetate using yeast
11. Resolution of S-ethyl hydroxybutonate using 3,5 dinitrobenzoate.
12. Preparation of 5,10,15,20-tetrakisphenyl porphyrin.
TOTAL: 60 PERIODS
REFERENCE
1. Fummis B.S., Hannaford A.J., Smith P.W.G., “Text Book of Practical Organic
Chemistry”, Longman Edition, 1995.
EQUIPMENTS / APPARATUS REQUIREMENTS
Heating Mantles (Nos. 5) / Water baths (Nos. 5) / Bunsen Burners (Nos. 15)
Round bottom flasks of various volumes (100ml, 500 ml, 250 ml – Nos.5)
condensers (Nos. 5), Distillation units (Nos. 2).
Reagents and consumables.
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