BT2201 CELL BIOLOGY SYLLABUS | ANNA UNIVERSITY B.TECH. BIOTECHNOLOGY 3RD SEMESTER SYLLABUS REGULATION 2008 2011-2012 BELOW IS THE ANNA UNIVERSITY THIRD SEMESTER B.TECH. BIOTECHNOLOGY DEPARTMENT SYLLABUS IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011-2012 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI,TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT2201 CELL BIOLOGY L T P C
3 0 0 3
MAIN AIM(S) OF THE COURSE
The course aims to develop skills of the Students in the area of Cell Biology and Cell
Signalling pathways. This will be necessary for studies in course like Microbiology,
Molecular course is also a prerequisite for other Biology, etc., This courses offered in the
subsequent semesters.
UNIT I CELL STRUCTURE AND FUNCTION OF THE ORGANELLES 9
Eukaryotic and prokaryotic cells, principles of membrane organisation, membrane
proteins, cytoskeletal proteins, types of cell division, mitosis & meiosis, extra cellular
matrix, cell cycle and molecules that control cell cycle.
UNIT II TRANSPORT ACROSS CELL MEMBRANES 9
Passive & active transport, permeases, sodium potassium pump, Ca2+ ATPase pumps,
lysosomal and vacuolar membrane ATP dependent proton pumps, co transport symport,
antiport, transport into prokaryotic cells, endocytosis and exocytosis. Entry of viruses
and toxins into cells.
UNIT III RECEPTORS AND MODELS OF EXTRA CELLULAR SIGNALLING 9
Cytosolic, nuclear and membrane bound receptors, examples of receptors, autocrine,
paracrine and endocrine models of action, quantitation and characterisation of receptors.
UNIT IV SIGNAL TRANSDUCTION 9
Signal amplification, different models of signal amplifications, cyclic amp, role of inositol
phosphates as messengers, biosynthesis of inositol tri phosphates, cyclic GMP and g
proteins, role in signal transduction, calcium ion flux and its role in cell signaling, current
models of signal amplification, phosphorylation of protein kinases, regulation of protein
kinases, serine –threonine kinases, tumor necrosis factor receptor families.
UNIT V CELL CULTURE 9
Techniques for the propagation of eukaryotic and prokaryotic cells. Cell line, generation
of cell lines, maintenance of stock cells, characterization of cells, immunochemistry,
morphological analysis techniques, in cell culture, ex-plant cultures primary cultures,
contamination, differentiation, three dimensional cultures, role of matrix in cell growth.
TOTAL : 45 PERIODS
TEXTS BOOKS
1. Darnell J, Lodish H, Baltimore D, “Molecular Cell Biology”, W.H.Freeman;
2. Kimball T.W., “Cell Biology”, Wesley Publishers;
REFERENCES:
1. De Robertis & De Robertis, “Cell Biology”.
2. James D.Watson, “Molecular Biology of the Cell”.
BT2201 CELL BIOLOGY L T P C
3 0 0 3
MAIN AIM(S) OF THE COURSE
The course aims to develop skills of the Students in the area of Cell Biology and Cell
Signalling pathways. This will be necessary for studies in course like Microbiology,
Molecular course is also a prerequisite for other Biology, etc., This courses offered in the
subsequent semesters.
UNIT I CELL STRUCTURE AND FUNCTION OF THE ORGANELLES 9
Eukaryotic and prokaryotic cells, principles of membrane organisation, membrane
proteins, cytoskeletal proteins, types of cell division, mitosis & meiosis, extra cellular
matrix, cell cycle and molecules that control cell cycle.
UNIT II TRANSPORT ACROSS CELL MEMBRANES 9
Passive & active transport, permeases, sodium potassium pump, Ca2+ ATPase pumps,
lysosomal and vacuolar membrane ATP dependent proton pumps, co transport symport,
antiport, transport into prokaryotic cells, endocytosis and exocytosis. Entry of viruses
and toxins into cells.
UNIT III RECEPTORS AND MODELS OF EXTRA CELLULAR SIGNALLING 9
Cytosolic, nuclear and membrane bound receptors, examples of receptors, autocrine,
paracrine and endocrine models of action, quantitation and characterisation of receptors.
UNIT IV SIGNAL TRANSDUCTION 9
Signal amplification, different models of signal amplifications, cyclic amp, role of inositol
phosphates as messengers, biosynthesis of inositol tri phosphates, cyclic GMP and g
proteins, role in signal transduction, calcium ion flux and its role in cell signaling, current
models of signal amplification, phosphorylation of protein kinases, regulation of protein
kinases, serine –threonine kinases, tumor necrosis factor receptor families.
UNIT V CELL CULTURE 9
Techniques for the propagation of eukaryotic and prokaryotic cells. Cell line, generation
of cell lines, maintenance of stock cells, characterization of cells, immunochemistry,
morphological analysis techniques, in cell culture, ex-plant cultures primary cultures,
contamination, differentiation, three dimensional cultures, role of matrix in cell growth.
TOTAL : 45 PERIODS
TEXTS BOOKS
1. Darnell J, Lodish H, Baltimore D, “Molecular Cell Biology”, W.H.Freeman;
2. Kimball T.W., “Cell Biology”, Wesley Publishers;
REFERENCES:
1. De Robertis & De Robertis, “Cell Biology”.
2. James D.Watson, “Molecular Biology of the Cell”.
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