BT 2209 CELL BIOLOGY LABORATORY SYLLABUS | ANNA UNIVERSITY B.TECH. BIOTECHNOLOGY 3RD SEMESTER SYLLABUS REGULATION 2008 2011-2012 BELOW IS THE ANNA UNIVERSITY THIRD SEMESTER B.TECH. BIOTECHNOLOGY DEPARTMENT SYLLABUS IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011-2012 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI,TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT 2209 CELL BIOLOGY LABORATORY L T P C
0 0 4 2
AIM(S) OF THE COURSE
The course aims is offering hands on training in the area of Cell culture and Cell
identification. This will serve as a prerequisite for post graduate and specialized studies
& research.
EXPERIMENTS
1. Introduction to principles of sterile techniques and cell propagation.
2. Principles of microscopy, phase contrast and fluorescent microscopy.
3. Identification of given plant, animal and bacterial cells and their components by
microscopy,
4. GRAM’S Staining,
5. Leishman Staining,
6. Thin Layer Chromatography,
7. Giemsa Staining,
8. Separation of Peripheral Blood Mononuclear Cells from blood,
9. Osmosis and Tonicity,
10. Tryphan Blue Assay,
11. Staining for different stages of mitosis in AlliumCepa (Onion).
TOTAL : 60 PERIODS
29
REFERENCE
1. Laboratory Investigations in Cell and Molecular Biology, Allen Bregman Wiley, 2001.
EQUIPMENTS / APPARATUS
Microbiological Hood for sterilization with UV lighting (One).
Bunsen Burners – 10 Nos.
Orbital Shaker and Incubator – 2 Nos.
Refrigerator – 1 No.
Reagents and consumables – Required amount.
BT 2209 CELL BIOLOGY LABORATORY L T P C
0 0 4 2
AIM(S) OF THE COURSE
The course aims is offering hands on training in the area of Cell culture and Cell
identification. This will serve as a prerequisite for post graduate and specialized studies
& research.
EXPERIMENTS
1. Introduction to principles of sterile techniques and cell propagation.
2. Principles of microscopy, phase contrast and fluorescent microscopy.
3. Identification of given plant, animal and bacterial cells and their components by
microscopy,
4. GRAM’S Staining,
5. Leishman Staining,
6. Thin Layer Chromatography,
7. Giemsa Staining,
8. Separation of Peripheral Blood Mononuclear Cells from blood,
9. Osmosis and Tonicity,
10. Tryphan Blue Assay,
11. Staining for different stages of mitosis in AlliumCepa (Onion).
TOTAL : 60 PERIODS
29
REFERENCE
1. Laboratory Investigations in Cell and Molecular Biology, Allen Bregman Wiley, 2001.
EQUIPMENTS / APPARATUS
Microbiological Hood for sterilization with UV lighting (One).
Bunsen Burners – 10 Nos.
Orbital Shaker and Incubator – 2 Nos.
Refrigerator – 1 No.
Reagents and consumables – Required amount.
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